The HUSERMET Project
the human serum metabolome in health and disease...

    

GC-MS


GC-MS: A Gas Chromatograph (GC) coupled to a Mass Spectrometer (MS).


Gas Chromatography:

  • Samples are injected onto a chromatography column and swept through by a constant flow of gas
  • As the compounds travel through the column they become caught up in the mesh
  • Different metabolites interact with this mesh in different ways which affects their movement along the column and allows them to be separated
  • This makes the metabolites easier to detect by mass spectrometry since they are not all released (eluted) at the same time


This technique is very useful for analysing small molecules.

  • It can be used to identify fatty acids, amino acids, organic acids and sugars in urine and blood and is used by clinicians to diagnose metabolic disorders
    • e.g. phenylketonuria (PKU) diagnosis involves the measurement of levels of phenylalanine and tyrosine amino acids

 

The standard operating procedure (SOP) used for GCMS analysis in the HUSERMET study is available here. If you use this SOP, please cite the following publications:

News
[25 Sep. 2012]
HUSERMET represented at Metabomeeting 2012
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[30 Jun. 2011]
New Publication in Nature Protocols
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[23 Jan. 2010]
Manchester Researcher Appointed New 'Dementia Tsar'
[Read More...]
[04 Jan. 2010]
Newly Appointed Data Analyst
[Read More...]
[01 Nov. 2009]
Recruitment Finished
[Read More...]
[01 Aug. 2009]
Two New Publications
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[14 Apr. 2009]
Newly Appointed Research Technician
[Read More...]
[19 Nov. 2008]
New Project Manager
[Read More...]
[12 Sept. 2008]
HUSERMET Researchers Look to the Metabolome for Answers
[Read More...]
[07 Jul. 2008]
The University of Manchester and GSK Strike New Research Alliance
[Read More...]
[07 Jul. 2008]
Prof Doug Kell is the New Chief Executive for BBSRC
[Read More...]
[10 Dec. 2007]
The Maturing Field of Metabolomics is Taking Steps Toward Guiding Personalized Nutrition
[Read More...]
[24 Aug. 2007]
Free Health 'MOTs' in World's Biggest Male Ageing Study
[Read More...]
[20 Feb. 2007]
Newly Appointed Bioanalytical Scientist for the HUSERMET Project
[Read More...]